Microsatellite analysis
Indication
Microsatellites consist of DNA sequences, each 1 – 6 nucleotides long,
which are repeated many times. The number of repeats is highly variable, making it possible to use microsatellites as markers in genetic tests. Microsatellites are generally found in the non-coding regions of the genome. Individuals typically have two alleles for all microsatellites, one inherited from each parent.
Microsatellite alleles normally differ slightly (i.e., they are heterozygous). If one of a pair of alleles is mutated or deleted or if there is loss of part or all of a chromosome, then loss of heterozygosity (LOH) occurs. Such LOH of microsatellite alleles on chromosome 3 in uveal melanoma cells indicates an increased risk of metastatic disease.
Microsatellites consist of DNA sequences, each 1 – 6 nucleotides long,
which are repeated many times. The number of repeats is highly variable, making it possible to use microsatellites as markers in genetic tests. Microsatellites are generally found in the non-coding regions of the genome. Individuals typically have two alleles for all microsatellites, one inherited from each parent.
Microsatellite alleles normally differ slightly (i.e., they are heterozygous). If one of a pair of alleles is mutated or deleted or if there is loss of part or all of a chromosome, then loss of heterozygosity (LOH) occurs. Such LOH of microsatellite alleles on chromosome 3 in uveal melanoma cells indicates an increased risk of metastatic disease.
Methodology
Multiplex PCR based analysis of extracted DNA
Test Parameters
Detects LOH of four microsatellites located on chromosome 3p and four microsatellites located on chromosome
3q
Sample requirements
Fresh biopsy or frozen uveal melanoma material together with a matched whole blood sample (100µl). MGG stained cytopsin or other histological slide or copy of the corresponding pathology report. This procedure requires at least 20ng DNA.
Contact
We are happy to process samples from external centres for MSA, please email mailto:hkalirai@liv.ac.uk for further information, or call: (+44) 0151 794 9117.
Multiplex PCR based analysis of extracted DNA
Test Parameters
Detects LOH of four microsatellites located on chromosome 3p and four microsatellites located on chromosome
3q
Sample requirements
Fresh biopsy or frozen uveal melanoma material together with a matched whole blood sample (100µl). MGG stained cytopsin or other histological slide or copy of the corresponding pathology report. This procedure requires at least 20ng DNA.
Contact
We are happy to process samples from external centres for MSA, please email mailto:hkalirai@liv.ac.uk for further information, or call: (+44) 0151 794 9117.
