Molecular pathology services provided
Multiplex ligation-dependent probe amplification (MLPA)
Multiplex ligation-dependent probe amplification (MLPA) is a multiplex PCR-based technique that detects changes in the copy number of genes. The P027 MLPA (MRC-Holland) assay is designed specifically to detect the common gross chromosomal abnormalities found in uveal melanoma of chromosomes 1, 3, 6 and 8. MLPA is used routinely for prognostic testing of uveal melanoma specimens not only for patients treated at the LOOC but also when requested by clinicians from other hospitals. Through our research we have shown that MLPA has increased sensitivity of detecting those patients at high risk of developing metastasis, compared to alternative methodologies such as fluorescence in situ hybridisation.
If you would like to submit a uveal melanoma specimen for MLPA testing please contact either Dr. M Baudo or Dr. H Kalirai for specific information about how the sample should be sent and the cost for this service.
MLPA Publications
Russo A, Rene C, Coupland SE, Sagili S, Damato B. Discrepancy between fluorescence in situ hybridization and multiplex ligation-dependent probe amplification in orbital recurrence of uveal melanoma 26 years after enucleation. Ophthal Plast Reconstr Surg. 2012 Nov;28(6):e140-2.
Lake SL, Kalirai H, Dopierala J, Damato BE, Coupland SE.Comparison of formalin-fixed and snap-frozen samples analysed by multiplex ligation-dependent probe amplification for prognostic testing in uveal melanoma. Invest Ophthalmol Vis Sci. 2012 Mar 16.
Callejo SA, Dopierala J, Coupland SE, Damato B. Sudden growth of a choroidal melanoma and multiplex ligation-dependent probe amplification findings suggesting late transformation to monosomy 3 type. Arch Ophthalmol. 2011 Jul;129(7):958-60. PubMed PMID: 21746991.
Lake SL, Jmor F, Dopierala J, Taktak AF, Coupland SE, Damato BE. Multiplex ligation-dependent probe amplification of conjunctival melanoma reveals common BRAF V600E gene mutation and gene copy number changes. Invest Ophthalmol Vis Sci. 2011 Jul 29;52(8):5598-604. Print 2011 Jul. PubMed PMID: 21693616.
Lake SL, Damato BE, Dopierala J, Baudo MM, Taktak AF, Coupland SE. Multiplex ligation-dependent probe amplification analysis of uveal melanoma with extraocular extension demonstrates heterogeneity of gross chromosomal abnormalities.Invest Ophthalmol Vis Sci. 2011 Jun 8.
Biswas A, Hamid B, Coupland SE, Franks A, Leonard N. Multiple periocular adult onset xanthogranulomas in a patient with chronic lymphocytic leukaemia. Eur J Dermatol. 2010 Mar-Apr;20(2):211-3. Epub 2009 Nov 17. PubMed PMID: 19919911.
Damato BE, Dopierala JA, Coupland SE. Genotypic profiling of 452 choroidal melanomas with Multiplex Ligation-Dependent Probe Amplification. Clin Cancer Res. 2010 Oct 25. [Epub ahead of print]
Dopierala J, Damato BE, Lake SL, Taktak AF, Coupland SE. Genetic heterogeneity in uveal melanoma assessed by multiplex ligation-dependent probe amplification. Invest Ophthalmol Vis Sci. 2010 May 19. [Epub ahead of print]
If you would like to submit a uveal melanoma specimen for MLPA testing please contact either Dr. M Baudo or Dr. H Kalirai for specific information about how the sample should be sent and the cost for this service.
MLPA Publications
Russo A, Rene C, Coupland SE, Sagili S, Damato B. Discrepancy between fluorescence in situ hybridization and multiplex ligation-dependent probe amplification in orbital recurrence of uveal melanoma 26 years after enucleation. Ophthal Plast Reconstr Surg. 2012 Nov;28(6):e140-2.
Lake SL, Kalirai H, Dopierala J, Damato BE, Coupland SE.Comparison of formalin-fixed and snap-frozen samples analysed by multiplex ligation-dependent probe amplification for prognostic testing in uveal melanoma. Invest Ophthalmol Vis Sci. 2012 Mar 16.
Callejo SA, Dopierala J, Coupland SE, Damato B. Sudden growth of a choroidal melanoma and multiplex ligation-dependent probe amplification findings suggesting late transformation to monosomy 3 type. Arch Ophthalmol. 2011 Jul;129(7):958-60. PubMed PMID: 21746991.
Lake SL, Jmor F, Dopierala J, Taktak AF, Coupland SE, Damato BE. Multiplex ligation-dependent probe amplification of conjunctival melanoma reveals common BRAF V600E gene mutation and gene copy number changes. Invest Ophthalmol Vis Sci. 2011 Jul 29;52(8):5598-604. Print 2011 Jul. PubMed PMID: 21693616.
Lake SL, Damato BE, Dopierala J, Baudo MM, Taktak AF, Coupland SE. Multiplex ligation-dependent probe amplification analysis of uveal melanoma with extraocular extension demonstrates heterogeneity of gross chromosomal abnormalities.Invest Ophthalmol Vis Sci. 2011 Jun 8.
Biswas A, Hamid B, Coupland SE, Franks A, Leonard N. Multiple periocular adult onset xanthogranulomas in a patient with chronic lymphocytic leukaemia. Eur J Dermatol. 2010 Mar-Apr;20(2):211-3. Epub 2009 Nov 17. PubMed PMID: 19919911.
Damato BE, Dopierala JA, Coupland SE. Genotypic profiling of 452 choroidal melanomas with Multiplex Ligation-Dependent Probe Amplification. Clin Cancer Res. 2010 Oct 25. [Epub ahead of print]
Dopierala J, Damato BE, Lake SL, Taktak AF, Coupland SE. Genetic heterogeneity in uveal melanoma assessed by multiplex ligation-dependent probe amplification. Invest Ophthalmol Vis Sci. 2010 May 19. [Epub ahead of print]
